I am an amateur user of Galaxy and have been trying to convert a bedgraph file generated from the Methyldackel tool into a bigwig format for visualisation using the Wig/Bedgraph - to - Bigwig converter tool. I am receiving this error message -
“Unrecognized line 1 of stdin:
chr1 3000826 3000828 80 4 1”
Can you please tell me what is wrong and how I can solve it?
could you share your Galaxy history with me? I’ll have a look at it. My email is
Thanks for replying.
I have shared the history with you. Kindly check if you are able to access it.
Hello @gallardoalba ,
Did you have a chance to look into this problem?
sorry, I wasn’t able to fix it yet. I’ll try to work on it throughout the week.
the problem was that the converter requires a BedGraph with only four columns; so you in your case you need to generate three different bedgraph files, one for each track.
Test history: Galaxy | Accessible History | imported: MIWI2 WGBS_abi
Sorry for the late solution, regards!