Hello,
I want to ask if galaxy has lettuce reference genome. I’m considering using galaxy for my RNA-seq project in lettuce. Thank you
Welcome, @Kelvin
You will need to find the assembled reference genome, and any available reference annotation for it, and load those files into Galaxy. You can then use the genome as a Custom Reference Genome with tools. This works the same as an index natively available on the server.
Start here → FAQ: How to use Custom Reference Genomes?
Similar Q&A at this forum involving that functionality can be found at the custom-genome tag.
And we have tutorials here → Transcriptomics / Tutorial List
From just a quick search I found the genome resource here → Lactuca sativa genome assembly Lsat_Salinas_v11 - NCBI - NLM
As someone who interned at the USDA in Salinas, CA 30 years ago, attempting to sequence the Lactuca sativa genome via PCR, your question made me smile!! Happy science! 
I usually don’t do this, but as a test and example, I copied the correct URLs from the FTP site (link is in a tab at the top) into a history using the Upload tool with all defaults. I then standardized the format of both so that these will work with any tool. I tagged everything, and those cleanup steps are in our FAQs (and prior topics!). Looks good to me!
Shared history (click on it to view, and import if you want to!) → https://usegalaxy.org/u/jen-galaxyproject/h/lactuca-sativa
You can add the other files if you want to, but these two are your baseline.
Hope this helps! 
Hello Jennifer,
Thank you so much. I appreciate it.
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