Hello,
I am trying to run a dataset of DIA data through DIA-NN. The tool gives me a “command not found” error. Below is the command which it runs and the error produced:
mkdir ./input_data && mkdir ./tmp && ln -s '/data/dnb09/galaxy_db/files/2/4/a/dataset_24a41ce2-079d-4ec4-bae4-893829868c10.dat' './input_data/191023JAT12_4_Mix_3_1ug_pOT_30k_390_1010_12.raw' && ln -s '/data/dnb09/galaxy_db/files/f/7/b/dataset_f7bda34f-ded0-44b5-bd73-c8eda435e3ad.dat' './input_data/191023JAT11_3_Mix_1_1ug_pOT_30k_390_1010_12.raw' && ln -s '/data/dnb09/galaxy_db/files/0/9/5/dataset_09577774-b359-4c6d-9c39-ef7a90882d8b.dat' './input_data/191023JAT10_2_Mix_4_1ug_pOT_30k_390_1010_12.raw' && ln -s '/data/dnb09/galaxy_db/files/8/6/6/dataset_866480a6-14d1-487a-8ce0-4e46a5becddd.dat' './input_data/191023JAT03_1_Mix_2_1ug_pOT_30k_390_1010_12%.raw' && diann '--f ./input_data/191023JAT12_4_Mix_3_1ug_pOT_30k_390_1010_12.raw--f ./input_data/191023JAT11_3_Mix_1_1ug_pOT_30k_390_1010_12.raw--f ./input_data/191023JAT10_2_Mix_4_1ug_pOT_30k_390_1010_12.raw--f ./input_data/191023JAT03_1_Mix_2_1ug_pOT_30k_390_1010_12%.raw' --dir ./ --lib '/data/dnb09/galaxy_db/files/7/a/3/dataset_7a34d44d-cdca-4f3f-aac9-46a68073595c.dat' --out ./report.tsv --threads ${GALAXY_SLOTS:-1} --temp ./tmp '--fasta /data/dnb09/galaxy_db/files/2/e/6/dataset_2e65db42-2e63-4486-b2aa-f9c419c45838.dat ' --relaxed-prot-inf --peak-center --no-ifs-removal --smart-profiling --cut 'K*,R*' --missed-cleavages '1' --met-excision --min-pep-len '7' --max-pep-len '30' --min-pr-mz '300' --max-pr-mz '1800' --min-pr-charge '1' --max-pr-charge '4' --min-fr-mz '200' --max-fr-mz '1800' --unimod4 --matrices --qvalue 0.01 --verbose '1' && cp ./report.tsv '/data/jwd05e/main/063/748/63748624/outputs/dataset_574b73bd-b8bf-470b-9afb-b6cfde5281c7.dat'
Error
/data/jwd05e/main/063/748/63748624/tool_script.sh: line 9: diann: command not found
I have run that dataset through EncyclopeDIA tool and it works fine in giving me an output as expected. I have submitted a bug report but would appreciate any help w.r.t resolving the issue.
Thanks