No piRNA or other antisense small RNA in Block Cluster Test?

  1. When I perform Block Cluster Test with my small RNA-seq data, I only see miRNA, tRNA and some sno RNA but no piRNA or other smallRNA. Does anybody know why?

Welcome, @Wenjing

Is this the tutorial you are following? Small Non-coding RNA Clustering using BlockClust. Tutorial data is a sub-sample of full data and while attempts are made to provide representative sub-samples, that isn’t always perfect.

Maybe the publication linked at the bottom of the tool form will help more? It is available open source. BlockClust: efficient clustering and classification of non-coding RNAs from short read RNA-seq profiles | Bioinformatics | Oxford Academic

Thanks a lot! I will have a look.