RNA STAR length of the genomic sequence around annotated junctions

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1

Hi, I’m new to RNA STAR and this may be a silly question.
How can we determine “Length of the genomic sequence around annotated junctions” for our samples?

Thanks for any help.

2

Hi @Danni
RNA_STAR has the following description: Used in constructing the splice junctions database. Ideal value is ReadLength-1 (–sjdbOverhang)
Hope that helps.
Kind regards,
Igor

3

Hi Igor,

My sample is paired-end 2x150bp, so that means the Length of the genomic sequence around annotated junctions should be 149?