Hello @moonmoondeb
For how to include your own genome for use with tools, please see the guides below.
With that context, to answer your question here
The solution would be to create a Custom Build database key for your genome, and to assign that to your input datasets. This will allow the tool to access the fasta index for your genome (to interpret scientific options like Effective genome fraction).
Be sure to carefully read that guide: format your genome fasta in a simple way, and create a unique database key that is all oneWord with no special characters, as an example GCA016699485 should work fine.
Do not reuse any existing server database key or expect problems.
For related topics here at this forum, including more help with troubleshooting how these work, please see custom-genome and custom-build .
Any questions you may have, please ask and we’ll try to help more.