Hello everyone I have a doubt, why do I have to input two FASTQ files from NCBI when I only have to align one read against the reference genome?
Which tool do you want to use to align? And do you already have a FASTQ file? Is it possible that your single fastq dataset is representing a paired-end run? If that is the case you can look at the tool named FASTQ splitter.
Maybe this page can also help:
After this step of uploading one SRA file in GALAXY
Why do we have to select two SRA accessions in the patterns field?