Using RiboGalaxy Remove ncRNA tool


I tried using the ribogalaxy bowtie tool to remove ncRNA however, after the job was run I couldn’t find any new files other than the mapping statistics for my data.

The mapping statistics said that 98% of the reads had not aligned (mean 2% were ncRNA) but then there was no new file in my history.

Would anyone be able to help me?



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Cross-posted here: Contact RiboGalaxy Support

Just a guess – but Bowtie2 as wrapped for Galaxy at other servers has an option to output unaligned reads. If that is what you were trying to do, maybe check the tool form to see if that was used or not.