bowtie2 problem

Hi everyone,

I’m not very experienced with bioinformatics, and I am running into a lot of trouble trying to align raw reads from Anna’s Hummingbird to the Anna’s Hummingbird genome using bowtie2 in Galaxy. I’ve already gotten them into fastqsanger format and trimmed the files. Every time, I get the following error: bowtie2 This job was terminated because it used more memory than it was allocated. Please click the bug icon to report this problem if you need help.

I’ve tried several different times and continue to receive this error. I am using the default settings. I would appreciate any and all help. Thanks.

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Hi Selasphoruskershaw,
I would suggest you to try selecting “Fast Local” under Do you want to use presets?
Also give it a try using raw files that are not trimmed.