I tried to analyze RNA-seq data successfully. Unfortunately, after running the DeSEQ2 tool I get the following error:
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
estimating size factors
gene-wise dispersion estimates: 6 workers
final dispersion estimates, MLE betas: 6 workers
Error in (function (cond) :
error in evaluating the argument ‘args’ in selecting a method for function ‘do.call’: BiocParallel errors
3 remote errors, element index: 1, 2, 3
0 unevaluated and other errors
first remote error: error in evaluating the argument ‘x’ in selecting a method for function ‘t’: no right-hand side in ‘b’
Calls: DESeq → DESeqParallel → do.call → bplapply → bplapply
At the same I can use edgeR and Lima without any problem.
I just wanted to compare the three different tool’s results!!
Does anybody know about it?