DeSeq2 Fatal Error - US.UTF-8 cannot be honored

[inivasDL]

Dear everyone,

I tried to analyze RNA-seq data successfully. Unfortunately, after running the DeSEQ2 tool I get the following error:

Warning message:
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
estimating size factors
estimating dispersions
gene-wise dispersion estimates: 6 workers
mean-dispersion relationship
final dispersion estimates, MLE betas: 6 workers
Error in (function (cond) :
error in evaluating the argument ‘args’ in selecting a method for function ‘do.call’: BiocParallel errors
3 remote errors, element index: 1, 2, 3
0 unevaluated and other errors
first remote error: error in evaluating the argument ‘x’ in selecting a method for function ‘t’: no right-hand side in ‘b’
Calls: DESeq → DESeqParallel → do.call → bplapply → bplapply

At the same I can use edgeR and Lima without any problem.
I just wanted to compare the three different tool’s results!!

Does anybody know about it?

Ini

[DCEM_NCU]

Hello I have the same message could you solve it?

[igor]

Hi @inivasDL , hi @DCEM_NCU
I realized it is way too late, but came across a similar error recently. In that case the issue was caused by genes with zero counts in every sample. Filtering out zero count genes helps. Alternatively, try other tools, such as edgeR.
Kind regards,
Igor