Error in DEG and Signal after de novo assembly

Hi everyone
I follow this assembly and differential expression of de novo transcriptomes step by step and at the Differential expression analysis, I provide everything as explained but every time I get error and not outcome generated. I’m suspicious of my Describe samples file, but it’s really simple and no error can be made.
Also, in SignalP 3.0, it limit to 4000 while my transcript it 72,000 lines, so it gives error, how you deal with this?
If you have any opinions on these please let me know.