I am new to Galaxy. I have generated a number of BAM files (mouse mm10) and started running them through feature counts. The 1st file ran fine using the in-built mm10 genome in featurecounts. Trying to run subsequent BAM files, now suddenly built-in genome is not available (see screen shot). Any ideas? Is this a bug or am I doing something wrong?
Thanks!
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Welcome @malbert
Check your inputs – is the metadata “database” mm10 assigned?
If not, then back up and check your mapping steps. Was the mapping actually against mm10? Was the same mapping tool/version used for all? In Galaxy or were these uploaded BAM data?