Featurecounts error

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1

Hello,
I am working on RNA seq data to get the differential gene expression data of sulfate-reducing bacteria. My bioinformatic pipeline is raw read → fastqc → trimmomatic → hisat2 → featurecounts → deseq2. I could get results until hisat2. But when I upload the hisat2 BAM file for featurecounts, I am getting an error “An error occurred with this dataset:” I checked with three different genomes with their corresponding reference genomes and I am getting an error with the featurecounts. Could any one help me with this?

Thank you
Jawahar

2

Hi @jawaharrajk,
could you share your history with me? My email is Screenshot from 2022-04-03 16-52-09.

Regards

3

Hi @jawaharrajk,
as I told you by email, you need to use paired-end lists, instead of collections of paired-end reads.

Regards