Hi @YONG_JIA
The only way to use reference genomes that are not already indexed at Galaxy Main https://usegalaxy.org (or any other public Galaxy servers) is to use a custom reference genome from the history. Please be aware that for some cases, the custom genome will be too large to use successfully.
If your PC does not have enough resources, and the custom genome functions fail for memory reasons, then you’ll need to consider other alternatives, such as Cloudman/AWS. AWS does offer “Grant in Education” that can help to cover the commercial costs for storage/computational work.
FAQs: https://galaxyproject.org/support/
- Preparing and using a Custom Reference Genome or Build
- Mismatched Chromosome identifiers (and how to avoid them)
Galaxy Choices:
Related Q&A: Reference genome import from main to local server