Issues running dada from DADA2

I have a large data set of 302 shallow shotgun sequences from Illumina NextSeq 2000. I have been running through the DADA2 pipeline and continue to not be able to get dada to run. I have tried different instances and am currently trying it on usegalaxy.fr but have had the same issue on each instance. No error/bug message is generated. The job is just grey with an empty output. I am currently only running 8 samples through the pipeline so I expect it should work. I have made my usegalaxy.fr history shareable if I can send the link securely.

I also made the history on the usegalaxy.org instance shareable.

Hi @Laura_Peirson

If a dataset is gray, that means the job is queued. How this works:

Then, if you are looking for a protocol to follow, this is probably what you are looking for.

I just ran through that tutorial as a test, and everything was working. I used the input data and workflow without any changes to create this reference/example.

Maybe those two resources can help you understand what is going on in your own history? But if you would like feedback here instead, you will need to post the link for your shared history back here with your question about it.

Right now it just seems to be queued – be sure to not stop/restart a job repeatedly or it may never get a chance to run! :slight_smile:

Got it! Thanks.

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