Hello, I am using the web-based GALAXY and I converted FASTQ files to FASTA and I am attempting to construct a BLAST database from these newly formed FASTA files.
As I use the makeblastdb function in Galaxy, I get an error message saying “Job output not returned from cluster error”. Has anyone experienced this before? If so, I would appreciate any possible solutions to this.
Check the tool form settings. Example: “Molecule type of input” – that should be toggled to be “nucleotide”. Other settings should probably be left at the default.
If all that is correct, and the tool still fails, then it is too large and you may need to assemble the sequences before using them as a target BLAST database.