I’m trying to run STAR-fusion by using data from previous RNA STAR aligment and a plug’n play reference.
These were the settings:
STAR-Fusion
Dataset Information
Number: 484 Name: STAR-Fusion on data 477, data 90, and others: fusion_candidates.final Created: Sun Dec 20 00:02:25 2020 (UTC) Filesize: 0 bytes Dbkey: ? Format: tabular Job Information
Galaxy Tool ID: toolshed.g2.bx.psu.edu/repos/iuc/star_fusion/star_fusion/0.5.4-3+galaxy1 Galaxy Tool Version: 0.5.4-3+galaxy1 Tool Version: None Tool Standard Output: stdout Tool Standard Error: stderr Tool Exit Code: None History Content API ID: bbd44e69cb8906b575af0a8b89f94c6f Job API ID: bbd44e69cb8906b514b6ba106ce1ce07 History API ID: a7538c218afd56b8 UUID: de7435f9-f145-49e2-86e5-48c266dc7584 Tool Parameters
Input Parameter Value Use output from earlier STAR run or let STAR Fusion control running STAR use_chimeric Chimeric junction file from STAR (with STAR-Fusion settings) * 426: RNA STAR on data 398, data 395, and others: chimeric junctions Source for sequence to search history Select the reference genome (FASTA file) * 91: GRCh38.p13.genome.fa uncompressed Gene model (gff3,gtf) file for splice junctions and fusion gene detection * 90: gencode.v35.annotation.gtf.gz Result of BLAST±blastn of the reference fasta sequence with itself * 477: https://data.broadinstitute.org/Trinity/CTAT_RESOURCE_LIB/GRCh38_gencode_v33_CTAT_lib_Apr062020.plug-n-play.tar.gz Settings to use default Job Resource Parameters no Inheritance Chain
STAR-Fusion on data 477, data 90, and others: fusion_candidates.final
After 3 weeks waiting for the job to run, I got the following error:
Dataset Error Report
An error occurred while running the tool toolshed.g2.bx.psu.edu/repos/iuc/star_fusion/star_fusion/0.5.4-3+galaxy1 .
Unable to run job due to a misconfiguration of the Galaxy job running system. Please contact a site administrator.