The data does exist at NCBI’s SRA: GSM3476303: RA1: RA patient1; Homo sapiens; RNA-Seq - SRA - NCBI
Tool for reference:
Faster Download and Extract Reads in FASTQ format from NCBI SRA (Galaxy Version 2.10.9+galaxy0)
There are two possibilities:
Rule out usage problems: Check to make sure you entering the accession ID only into the tool form. Any extra content will cause problems. Note that it is possible to input a list of accessions (more than one). That list should be in tabular format and include one accession per line with no extra whitespace or tabs.
Test to see if there is a technical problem: NCBI may be busy at this time. Trying a rerun usually resolves this type of issue. If you keep having problems, you can copy the data URL directly from NCBI and paste that into the Upload tool as an alternative data retrieval method. Find the data link using the first link I posted above.
If a rerun still fails, please write back and note:
- Where you are working. The URL of a public Galaxy server or explain if some other deployment type.
- What full tool name including version you are using.
- Did copy/pasting the data URL work as an alternative?
- A screenshot of the tool form. Use the “rerun” double-circle icon to bring up the tool form as it was configured when the result was an error.
Note: NCBI is reorganizing its data into a new cloud storage platform. This impacts some accessions but not all, and which accessions are impacted changes over time. Galaxy cannot control or predict of that is what is going on … but there is usually a way to get around those transient data retrieval issues. Let’s eliminate tool usage/technical issues on the Galaxy side, and try the Upload workaround if needed, as first-pass solutions.
Let’s start there.