Hi, i have a 600bp sequence with erronous base calls in the middle due to low quality. How can i convert all base calls with a Phred score of lees than 30 to “N” ? I can trim the ends but i would like to filter the entire sequence.
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I think this tool is something to try:
- Manipulate FASTQ reads on various attributes (link at ORG)
Update! This one is easier…
- FASTQ Masker by quality score (link at ORG)
Let us know how that works! 