Hi, I am getting this error when trying to run RNA-STAR, how can I resolve it? Thank you. Fatal error: Matched on FATAL ERROR Transcriptome.cpp:48:Transcriptome: exiting because of INPUT FILE error: could not open input file /cvmfs/data.galaxyproject.org/managed/rnastar_index2/hg38/dataset_950901_…

Hello, You need to supply a reference annotation GFT dataset from the history at runtime. The GTF should be based on the UCSC “hg38” genome build. Some choices: For Gencode, copy the link to the GTF and paste it into the Upload tool. Hg38 data is here https://www.gencodegenes.org/ . After it i…

RNA-STAR and hg38 GTF reference annotation

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jennaj Split this topic December 19, 2022, 11:48pm 9

A post was split to a new topic: Human hg38 GTF, source Gencode

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RNA-STAR and hg38 GTF reference annotation

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