how to add normalization factor when using diffbind to do the peak difference analysis
Hi @ChenJ
Do you mean the dba.normalize()
function? That isn’t supported on the Galaxy form (yet).
However, you could move into an R environment from Galaxy, send your data over, and then run the Diffbind package directly in there.
The top sections of this tutorial include links to the R introduction tutorials we host. And maybe the other examples in here about loading packages help, too, even though you are doing something else? Once in the RStudio interactive-tools environment it will work the same as it would anywhere else. → Hands-on: RNA Seq Counts to Viz in R / RNA Seq Counts to Viz in R / Transcriptomics
Hope this helps!