MSstats tool error

Hello,
I have been trying to use MSStat tool in Galaxy, where I uploaded SRM data in MSstat format however, I got an error: cant assign 4 names to a 0 column data table.
How can I solve this? I also tried uploading skyline data with annotiation file but an error occured saying that check annotiation.
Thank you very much in advance

Welcome, @esinsahin

Your error message indicates a format problem with the input data, so start by double checking that with your run.

I’ve also started up a simple example in this history as a test → https://usegalaxy.eu/u/jenj/h/test-msstats

This tool has quite a bit of help but that might be a bit overwhelming to navigate! Thankfully we have several tutorials to help more. Please see → Galaxy Training Network (GTN) Tutorials for MSstats: statistical relative protein significance analysis in DDA, SRM and DIA Mass Spectrometry

Let’s start there, and please let us know if you solved this already :scientist:

Update

The test run completed, and I can generate the same error when the data file contains csv content but the assigned datatype format is tabular.

There are two ways to correct this (maybe is your conflict as well?).

Examples of all three runs are in the test history above, also here again https://usegalaxy.eu/u/jenj/h/test-msstats

Hopefully this helps but let us know. We can troubleshoot your job more too :slight_smile: