Starsolo: Spliced and unspliced matrices

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1

Hello!

I was wondering if it would be possible to add a parameter to the RNA STARsolo tool. The new option --soloFeatures Gene Velocyto from STAR 2.7.9a would allow quantification of spliced/unspliced reads.

Thanks!

Erik J

2

Hi @erik_jacques,
a Pull Request has been opened in order to include that functionality.

Regards

1 Like
3

Cool! Thanks!

4

Morning!

I see that there is an issue in the pull request. Is there anything I can do to help? Perhaps provide sample data?

5

Hi, the files that need to be provided are those inside the Velocyto folder, isn’t it? Regards

6

The function was released with STAR 2.7.9a (Galaxy uses 2.7.8a) and I don’t see a velocyto folder under the master branch so I’m not sure.