I am using Transit Gumbel tool to determine gene essentiality from my transposon libraries using the tutorial titled “Essential genes detection with Transposon Insertion sequencing”.
However, the Gene ID (“orf” column) that was generated does not exactly match the gene ID of my organism. The gene ID generated was listed as multiples of 5 (D7S_RS00010, D7S_RS00015, D7S_RS00020, etc).
- Any ideas on what have gone wrong?
- I suspected that maybe I uploaded the wrong “type” of file from NCBI, instead of the gene annotation file. How do you upload gene annotation file (in the gff3 format) from NCBI to your history?
Thank you all!