Hi Everyone,
I am working with a very niche insect species and I am trying to perform RNAseq analysis using SRA files from NCBI. I have gotten to the point in the RNAseq training where I now have to map the RNAseq files to the organismal genome. I have been able to retrieve the GTF file of the species genome from NCBI, and now I am trying to use RNAstar. My issue is that once I have selected : use genome reference without builtin gene-model but provide a gtf (as per the instructions provided by the tutorial) I am unable to find my species in the organism genome dropdown. I am prompted to contact the Galaxy team (–genomeDir), but I am also not sure how to do that so I am reaching out here in hopes that someone can help me with either finding out how to contact them, or being able to run RNAstar. Here is what my issue looks like :
I hope to hear from someone soon!
thank you all