I used a reference genome (2.7 gb) from NCBI to perform RNA-STAR.
However, when I ran RNA-STAR, I saw an error in the attached screenshot. My computer has 160GB of RAM. Is it possible to increase the limitGenomeGenerateRAM? or is there any other solution in my local galaxy?
EXITING because of FATAL PARAMETER ERROR: limitGenomeGenerateRAM=31000000000is too small for your genome
SOLUTION: please specify --limitGenomeGenerateRAM not less than 31128027402 and make that much RAM available
Hi @ohoh
The parameter is not in the tool form, since it is an administrative setting in the macros for the tool form itself. You can adjust that there in your own copy. Find it here → tools-iuc/tools/rgrnastar/macros.xml at df08c30df58d3e0db207386f64e7cca215dd8915 · galaxyproject/tools-iuc · GitHub
If you are using a custom genome, please know that creating new indexes for RNA STAR is the biggest memory surge in the tool suite! If you can at all source a pre-computed index, that would be strongly recommended. You can attach our Galaxy CVMFS resource to your local Galaxy.
If we don’t host the genome, you can try to create it yourself. The same macros file referenced above controls the Data Manager, too.
See the administrative tutorials here. Scroll down to the section → Data Management & Reference Data
And, I’m going to mention one more item: errors like your can come up due to data problems, too. So, double check your inputs! This forum has many prior topics where problem with reference data formats and other inputs went through troubleshooting. You can try a search with your error message, and any other parts of that message that seem informative.
Hope this helps and we can follow up! 
